Related Experiment Video

Updated: Jun 14, 2025

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.4K

Roles of the MO25 protein Pmo25 in contractile-ring stability and localization of the NDR kinase Sid2 during

Yanfang Ye1,2, Sha Zhang1, Aysha H Osmani1,3

  • 1Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.

Biorxiv : the Preprint Server for Biology
|June 4, 2025
PubMed

Insights

Mouse protein-25 (MO25) is vital for cell division. This study shows MO25 protein Pmo25 interacts with key proteins to ensure stable contractile rings and proper cell separation in fission yeast.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Mouse protein-25 (MO25) proteins are essential for development and morphogenesis across diverse organisms.
  • In fission yeast, Pmo25 is critical for maintaining cell polarity and division.
  • The precise role of Pmo25 in regulating cytokinesis is not well understood.

Purpose of the Study:

  • To elucidate the molecular mechanism by which Pmo25 regulates cytokinesis in fission yeast.
  • To identify proteins that interact with Pmo25 and contribute to its function in cell division.

Main Methods:

  • Yeast two-hybrid screening to identify interacting proteins.
  • Co-immunoprecipitation and in-vitro binding assays to confirm interactions.
  • Analysis of cytokinesis defects in pmo25 mutant strains.

Main Results:

  • Pmo25 directly binds to Ync13, a Munc13/UNC-13 family protein.
  • pmo25 mutants exhibit defective contractile-ring stability and septation, with rings sliding or disintegrating.
  • Pmo25 interacts with Cdc4, the myosin-II light chain, crucial for contractile-ring assembly.
  • Division-site recruitment of Sid2 kinase and Eng1 glucanase is impaired in pmo25 mutants.

Conclusions:

  • Pmo25 plays a novel role in regulating cytokinesis by stabilizing the contractile ring and ensuring proper recruitment of key division proteins.
  • The findings reveal a conserved mechanism for MO25 proteins in linking various cellular processes, including cell division.
Keywords:
Cdc4MO25Munc13/UNC-13SIN pathwaySid2Ync13contractile ringcytokinesisglucanase Eng1septum

More Related Videos

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

18.6K
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets10:52

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Published on: August 13, 2016

9.7K

Related Concept Videos

The Contractile Ring02:15

The Contractile Ring

Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
6.3K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K
The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
3.4K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.9K