Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Aryldiazonium Salts to Azo Dyes: Diazo Coupling01:11

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

3.1K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...
3.1K
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

1.8K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
1.8K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

7.4K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Molecular Structure to Macroscopic Performance: Insights into Polycarbosilane Curing.

Macromolecular rapid communications·2026
Same author

Adaptive cruise control for electric vehicles using hybrid-mode MPC.

Scientific reports·2026
Same author

A Benchmark and Basis-Set Extrapolation Study of Hyperfine Coupling Constants from the Random Phase Approximation and σ-Functionals.

The journal of physical chemistry. A·2026
Same author

A detailed comparison of ΔSCF methods with the constraint-based orbital-optimized excited state method.

Communications chemistry·2026
Same author

Machine learning models for smart grid stability prediction: a comparative analysis.

Scientific reports·2026
Same author

Chemoselective Alkylating Esterification of Thiophosphinic Acids: An Experimental and Theoretical Study.

ChemPlusChem·2026

Related Experiment Video

Updated: Sep 19, 2025

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

10.2K

A Platform for the Development of Highly Red-Shifted Azobenzene-Based Optical Tools.

Kyra Lützel1, Henryk Laqua2,3, Manjima B Sathian1

  • 1Department of Pharmacy, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, Munich, 81377, Germany.

Angewandte Chemie (International Ed. in English)
|June 4, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed new azobenzene photoswitches for optical tools. A combined experimental and computational approach enables the design of novel, red-shifted photoswitches with tunable properties for advanced applications.

Keywords:
Chemical probe developmentIn silico designPhotophysical propertiesRed‐shifted azobenzenes

More Related Videos

Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications
08:06

Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

Published on: June 2, 2017

14.2K
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.2K

Related Experiment Videos

Last Updated: Sep 19, 2025

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

10.2K
Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications
08:06

Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

Published on: June 2, 2017

14.2K
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.2K

Area of Science:

  • Organic Chemistry
  • Photochemistry
  • Computational Chemistry

Background:

  • Azobenzenes are crucial photoswitches for optical tools and photopharmaceuticals.
  • Designing azobenzene photoswitches with specific properties for targeted applications is challenging.

Purpose of the Study:

  • To develop synthetic protocols for dfdc (di-ortho-fluoro-di-ortho-chloro) azobenzene derivatives.
  • To investigate structure-property relationships, focusing on the n → π* absorption band.
  • To establish and validate a computational method for predicting UV-vis spectra.

Main Methods:

  • Synthesis of dfdc azobenzene derivatives with para-/ortho-position modifications.
  • Photophysical characterization using NMR, UV-vis, and X-ray analysis.
  • Computational modeling combining MD simulations and TD-DFT calculations.

Main Results:

  • 15 new visible light-operated photoswitches were synthesized with diverse relaxation rates.
  • Several derivatives exhibited enhanced bathochromic shifts compared to the lead dfdc azobenzene.
  • A validated computational approach for predicting UV-vis spectra of flexible azobenzenes was established.

Conclusions:

  • The study provides a foundation for in silico design of novel red-shifted photoswitches.
  • Dfdc azobenzenes show potential as chemical tools, exemplified by dfdc-OptoBI-1 activating TRPC6 channels.
  • This work advances the development of azobenzene-based optical devices and photopharmaceuticals.