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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Sep 19, 2025

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

Published on: April 20, 2009

Primer-Disk-Enabled DNA Data Storage System with Index and Record-Many-Read-Many Features.

Jiaxiang Ma¹, Yu Yang¹, Ben Pei¹

¹Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|June 4, 2025

Summary

This study introduces a novel DNA data storage system using a primer disk and QR codes for easy recording and retrieval. This hierarchical approach enables multiple DNA file storage and random access for practical DNA data applications.

Keywords:

DNA data storage inkjet printing micro‐fabrication record‐many‐read‐many memory

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Last Updated: Sep 19, 2025

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

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Published on: April 20, 2009

Databases to Efficiently Manage Medium Sized, Low Velocity, Multidimensional Data in Tissue Engineering

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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Area of Science:

Biotechnology
Information Science
Materials Science

Background:

DNA data storage offers high density but faces challenges in data structuring for efficient recording and retrieval.
Current methods lack robust systems for organizing and accessing multiple DNA-encoded files.

Purpose of the Study:

To develop a hierarchical DNA data storage system for simplified recording, retrieval, and reading.
To enable multiple immobilizations of DNA molecules and generate QR codes for indexing and access.

Main Methods:

A primer-disk system was engineered with multiple primers for on-demand covalent immobilization of DNA molecules via solid-phase PCR.
Encoded DNA files were linked to fluorescent QR codes for retrieval and indexing.
A primer disk supporting up to 10 primers was utilized for sequential data storage.

Main Results:

Demonstrated successful storage and retrieval of different DNA files on the primer disk.
Fluorescent QR codes provided accessible indexing for decoding information after imaging and recognition.
Recorded DNA files were randomly accessible and readable via solid-phase PCR up to 20 times.

Conclusions:

The developed primer-disk-enabled hierarchical DNA data storage system offers record-many-read-many capabilities.
This system simplifies DNA data management, paving the way for practical applications in DNA data storage.