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Related Concept Videos

Wood Panel Products01:18

Wood Panel Products

Wood panel products are essential materials used in construction for applications such as flooring, siding, and roofing, typically available in standard dimensions of 4 feet by 8 feet, with thicknesses varying from one-quarter of an inch to one and one-eighth inches. Among the most common types of wood panels is plywood, which is produced by gluing multiple layers of thin wood veneers under pressure. The grain of the outer veneers runs lengthwise, while the grains of the interior layers run...

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The 96-Puddle Paper Plate as a Versatile Lab-on-Paper Platform.

Taeha Lee1,2, Yeongjun Seo1, Dain Kang1

  • 1Department of Biotechnology and Bioinformatics, Korea University, Sejong, 30019, South Korea.

Small Methods
|June 4, 2025
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Summary
This summary is machine-generated.

A novel 96-Puddle Paper Plate (96-PPP) offers high-throughput analysis for diverse analytes. This lab-on-paper platform enhances reliability and versatility, overcoming limitations of traditional microfluidic paper-based analytical devices (µPADs).

Keywords:
amyloidcolorimetryfluorometryhigh‐throughput assaylab‐on‐paper

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Materials Science

Background:

  • Microfluidic paper-based analytical devices (µPADs) enable diverse analyte detection but face challenges in standardization and data reliability due to specialized equipment needs.
  • Conventional µPAD designs limit high-throughput analysis, universal application, and consistent data acquisition, hindering broader scientific adoption.

Purpose of the Study:

  • To introduce the 96-Puddle Paper Plate (96-PPP), a novel lab-on-paper platform designed for high-throughput analysis and compatibility with standard microplate readers.
  • To demonstrate the 96-PPP's versatility in detecting various analytes and its capability for advanced biological studies, addressing limitations of existing µPADs.

Main Methods:

  • Development of the 96-Puddle Paper Plate (96-PPP) as a scalable, cellulose-based sheet compatible with microplate readers.
  • Qualitative and quantitative analysis of analytes including acetone, magnesium ions, ascorbic acid, and ketone using visual inspection, smartphone imaging, absorbance, and fluorescence measurements.
  • Investigation of amyloid deposition and proteolysis within cellulose networks mimicking extracellular matrix structures.

Main Results:

  • The 96-PPP platform demonstrated high-throughput capabilities, enabling both qualitative and quantitative measurements for a range of analytes relevant to diagnostics and environmental monitoring.
  • Achieved consistent detection of analytes across 480 puddles with low variation (3.5%) for amyloid deposition studies.
  • Successfully replicated the functionality of 96-well plates while overcoming the limitations of conventional µPADs in terms of productivity, versatility, and reliability.

Conclusions:

  • The 96-Puddle Paper Plate (96-PPP) represents a significant advancement in lab-on-paper technology, offering a standardized and scalable solution for high-throughput analysis.
  • This versatile platform enhances the reliability and accessibility of µPADs, facilitating applications in clinical diagnostics, metabolic research, and environmental monitoring.
  • The 96-PPP overcomes key limitations of conventional µPADs, paving the way for broader adoption and more robust scientific discovery.