Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.0K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.0K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.5K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.5K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.6K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.6K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.7K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The Expression and Significance of Serum Protein ROCK2 in Patients with Chronic Graft-Versus-Host Disease].

Zhongguo shi yan xue ye xue za zhi·2021
Same author

[The Clinical Characteristics and Outcomes of the Patients with POEMS Syndrome].

Zhongguo shi yan xue ye xue za zhi·2021
Same author

Mental health care integration and primary care patient experience in the Veterans Health Administration.

Healthcare (Amsterdam, Netherlands)·2021
Same author

Polydopamine-based nanoplatform for photothermal ablation with long-term immune activation against melanoma and its recurrence.

Acta biomaterialia·2021
Same author

Co-delivery of autophagy inhibitor and gemcitabine using a pH-activatable core-shell nanobomb inhibits pancreatic cancer progression and metastasis.

Theranostics·2021
Same author

Step-by-step dual stimuli-responsive nanoparticles for efficient bacterial biofilm eradication.

Biomaterials science·2021

Related Experiment Video

Updated: Jan 18, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.6K

Phase Correction of MR Spectroscopic Imaging Data Using Model-Based Signal Estimation and Extrapolation.

Wen Jin, Rong Guo, Yudu Li

    IEEE Transactions on Bio-Medical Engineering
    |June 4, 2025
    PubMed
    Summary

    This study introduces a novel model-based method for phase correction in magnetic resonance spectroscopic imaging (MRSI) data. The new technique effectively generates high-quality absorption-mode spectra, outperforming existing methods in robustness and accuracy.

    More Related Videos

    NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
    08:54

    NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

    Published on: May 1, 2017

    26.8K
    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
    09:30

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

    Published on: January 18, 2017

    12.4K

    Related Experiment Videos

    Last Updated: Jan 18, 2026

    Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
    10:28

    Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

    Published on: November 2, 2018

    12.6K
    NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
    08:54

    NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

    Published on: May 1, 2017

    26.8K
    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
    09:30

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

    Published on: January 18, 2017

    12.4K

    Area of Science:

    • Magnetic Resonance Imaging and Spectroscopy
    • Biomedical Data Analysis
    • Signal Processing

    Background:

    • Phase correction is crucial for generating absorption-mode spectra in magnetic resonance spectroscopic imaging (MRSI).
    • Conventional methods struggle with low signal-to-noise ratio (SNR) and baseline distortions, leading to inaccurate spectral data.
    • Developing robust phase correction techniques is essential for reliable MRSI analysis.

    Purpose of the Study:

    • To develop and validate a novel model-based method for improved phase correction of MRSI data.
    • To address limitations of conventional phase correction methods, particularly in noisy conditions and with acquisition dead time.
    • To generate high-quality absorption-mode spectra from MRSI data for enhanced analysis.

    Main Methods:

    • A novel model-based approach was developed for MRSI phase correction.
    • The method utilizes a Lorentzian-based spectral model to determine zeroth-order phase and acquisition dead time.
    • Signal extrapolation is performed using a generalized series model, followed by absorption-mode spectra generation.

    Main Results:

    • The proposed method demonstrated improved parameter estimation accuracy in simulations across various noise levels and dead times.
    • High-quality absorption-mode spectra with minimal distortions were consistently generated from both simulated and experimental multi-nuclei MRSI data.
    • Comparative analysis showed superior robustness and reduced spectral distortion compared to state-of-the-art methods like the entropy and LCModel methods.

    Conclusions:

    • A novel and effective model-based method for MRSI phase correction has been successfully developed.
    • The method reliably produces high-quality absorption-mode spectra from simulated and in vivo MRSI data.
    • This technique offers a valuable tool for processing and analyzing MRSI data, improving diagnostic capabilities.