Lab on Pipette: Robust Biochemical Sensing with Functional Laser-Induced Graphene Electrode Array for On-Site and
Xianyou Sun1, Zihan Jin1, Shaoxiang Zhang1
1Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.
ACS Nano
|June 4, 2025
Summary
This study introduces a "lab on pipette" system for easy, on-site urine analysis. This portable sensor efficiently detects multiple disease biomarkers, improving personalized health monitoring.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Sensor Technology
Background:
- Urine is a rich source of disease biomarkers, ideal for noninvasive diagnostics.
- Portable biochemical sensors offer convenient on-site analysis but face challenges with complex samples and sensor reuse.
- Existing limitations hinder widespread, user-friendly urine analysis.
Purpose of the Study:
- To develop a novel "lab on pipette" (LOP) system for efficient, on-site urine analysis.
- To enable the detection of multiple urine biomarkers using a portable and disposable sensor.
- To overcome limitations of current sensors in complex sample handling and sensor renewal.
Main Methods:
- Fabrication of an electrochemical electrode array on flexible polyimide using laser-induced graphene (LIG) technology.
- Development of a multilayer functional interface using gold nanodendrites, Prussian blue (PB), and nickel hexacyanoferrate (NiHCF) for sensitive detection.
- Integration of nanoalumina-doped porous enzyme membranes for enhanced sensor reliability and repeatability.
Main Results:
- Highly sensitive detection of pH, hydrogen peroxide, glucose, and ascorbic acid with pH compensation.
- Achieved low detection limits of 7.19 μM for glucose and 8.57 μM for ascorbic acid.
- Demonstrated enhanced stability of PB via NiHCF doping and robust sensor performance on curved surfaces.
Conclusions:
- The LOP system provides a low-cost, disposable, and portable solution for on-site biochemical detection.
- The developed sensor exhibits high sensitivity, reliability, and potential for personalized health monitoring.
- This technology facilitates convenient and large-scale urine analysis for various diagnostic applications.
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