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Updated: May 11, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
A novel biosensor based on donor-π-acceptor polymer dots enabling stable cathodic electrochemiluminescence detection
Zhuang Chen1, Siyuan Ji1, Chenwei Gu1
1School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
Abstract:
Antibiotic residues in food and the environment pose a significant threat to public health, requiring advanced detection strategies. In this study, a novel D-π-A polymer nanoparticle (P-Pdots) was developed with cathodic electrochemiluminescence (ECL) performance comparable to the classical Ru(bpy)32+/TPrA system. The vinyl bond as a π bridge not only improved the degree of electron delocalization by extending the π-conjugation length, but effectively accelerated intramolecular charge transfer, thereby significantly enhancing the ECL signal. Transient ECL tests revealed the stability of P-Pdots radical ions and elucidated a reductive-oxidative (R-O) cathodic ECL mechanism. An ultrasensitive ECL resonance energy transfer (ECL-RET) biosensor for streptomycin detection was constructed based on the effective quenching effect of metal-organic-framework functionalized gold nanoparticles (NH2-MIL-53(Al)@Au) on P-Pdots. The biosensor exhibited a wide detection range (0.5 pM-200 nM) and low detection limit (120 fM), and was successfully applied to the detection of STR residues in real samples (milk, honey and Yangtze River water). This work provides new ideas for the design of efficient R-O type ECL luminophores, and establishes a sensitive and selective sensing platform with broad applicability in environmental and food safety monitoring.
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