Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrophilic albumin microspheres.

W E Longo, E P Goldberg

    Methods in Enzymology
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    A novel method synthesizes hydrophilic human serum albumin microspheres (HSA/MS) using glutaraldehyde cross-linking and polymer solutions. These versatile HSA/MS can be chemically modified for controlled drug release and hydrophobicity, enabling new therapeutic applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Non-operative management of acute cholecystitis in the elderly.

    The British journal of surgery·2012
    Same author

    Management of malignant pleural effusion by suicide gene therapy in advanced stage lung cancer: a case series and literature review.

    Cancer gene therapy·2012
    Same author

    EphrinA1 inhibits malignant mesothelioma tumor growth via let-7 microRNA-mediated repression of the RAS oncogene.

    Cancer gene therapy·2011
    Same author

    HPLC determination of dexamethasone in human plasma and its application to an in vitro release study from endovascular stents.

    Die Pharmazie·2006
    Same author

    Ischemic colitis.

    Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2005
    Same author

    Primary signet-ring cell carcinoma mimicking segmental Crohn's colitis.

    Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2005
    Same journal

    Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

    Methods in enzymology·2026
    Same journal

    A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

    Methods in enzymology·2026
    Same journal

    Evolutionary genetic approaches to analyze mucins.

    Methods in enzymology·2026
    Same journal

    Ex vivo imaging and enzymatic analysis of intestinal mucus.

    Methods in enzymology·2026
    Same journal

    Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

    Methods in enzymology·2026
    Same journal

    Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

    Methods in enzymology·2026
    See all related articles

    Area of Science:

    • Biomaterials Science
    • Drug Delivery Systems
    • Nanotechnology

    Background:

    • Developing stable, functionalizable microparticulate systems is crucial for advanced drug delivery.
    • Human serum albumin microspheres (HSA/MS) offer biocompatibility but require controlled synthesis for optimal performance.
    • Existing methods may lack versatility in surface modification and drug loading strategies.

    Purpose of the Study:

    • To develop a novel method for synthesizing unique hydrophilic human serum albumin microspheres (HSA/MS).
    • To demonstrate the versatility of these HSA/MS for chemical modification and controlled drug release.
    • To explore potential applications in drug delivery, immunotherapy, and diagnostics.

    Main Methods:

    • Synthesis of hydrophilic HSA/MS via glutaraldehyde cross-linking in an organic phase.

    Related Experiment Videos

  • Utilizing concentrated polymer solutions for steric stabilization of albumin microdispersions.
  • Surface modification of HSA/MS to introduce controlled hydrophobicity.
  • Covalent and physical binding of Adriamycin to HSA/MS.
  • In vitro drug release studies using a dynamic flow method.

    • Main Results:

    • Successfully prepared hydrophilic HSA/MS that disperse readily in aqueous media without surfactants.
    • Demonstrated that steric stabilization is dependent on polymer concentration and molecular weight.
    • Achieved controlled hydrophobicity through surface modification with fatty amines.
    • Bound up to 18 wt% Adriamycin via both physical association and covalent linkage.
    • Observed distinct drug release profiles: slow release from covalent bonds and fast release from physical adsorption.

    Conclusions:

    • The developed method provides a versatile platform for producing chemically modifiable hydrophilic HSA/MS.
    • These HSA/MS can encapsulate high concentrations of therapeutic agents with tunable release kinetics.
    • The ability to control hydrophobicity and drug release mechanisms opens new avenues for drug delivery, vaccine development, and immunodiagnostics.