Related Experiment Video
Updated: Mar 7, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
Insulin post-transcriptional regulation via PARP12-mediated ADP- ribosylation
Abstract:
ADP-ribosylation is a common modification that occurs in proteins and nucleic acids, regulating many cellular processes ranging from DNA repair to inflammatory signaling. ADP-ribosylation plays an important role in cancer biology, infectious diseases, and obesity, but its role in the development of type 1 diabetes is not well understood. Here, we studied the role of ADP-ribosyltransferase PARP12 in type 1 diabetes development. PARP12 expression is highly induced in human islets treated with pro-inflammatory cytokines or β cells from diabetic donors. Proteomics analysis of MIN6 insulin-producing cells identified that the RNA machinery is regulated by PARP12 during inflammation. PARP12 also ADP-ribosylates 150 mRNAs, including the insulin mRNA. This mRNA ADP-ribosylation in turn modifies transcript localization and halts translation. Overall, our data identified a role for PARP12 in ADP-ribosylation and translation halting of mRNAs, which may affect insulin production during insulitis.
More Related Videos
Related Concept Videos
Insulin: The Receptor and Signaling Pathways
Insulin: Biosynthesis, Chemistry, and Preparation
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment...
PI3K/mTOR/AKT Signaling Pathway
Insulin Secretory Vesicles
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Regulation of Expression at Multiple Steps

