Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

2.8K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.8K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

5.0K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.0K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

1.8K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
1.8K
Overview of Exosomes01:36

Overview of Exosomes

2.9K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.9K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.2K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Injectable Self-Healing Oxidized Hyaluronic Acid Hydrogel Remodels the Healing Microenvironment for Efficient Wound Closure.

Acta biomaterialia·2026
Same author

An Injectable Rapid-Adhesion and Self-Expanding Underwater Hydrogel Adhesive with Biodegradation and Reinforced Hemostasis for Deep Noncompressible Hemorrhage Management.

ACS biomaterials science & engineering·2026
Same author

Progress in the Application of Hydrogel Materials for the Treatment of Intestinal Fistula.

Macromolecular bioscience·2026
Same author

Intracavernous Injection of Mechanically Extracted Stromal Vascular Fragments Suppresses Endothelial-Mesenchymal Transformation to Mitigate Erectile Dysfunction in Hypertensive Rats.

Stem cells international·2026
Same author

Green Synthesis of Biobased NIPUrea-Acrylate Hybrids for Versatile, Fast-Curing Hot-Melt Coating Development.

ACS applied materials & interfaces·2025
Same author

Sphingolipid Metabolism Dysregulation Drives Immune Microenvironment Remodeling and Predicts Prognosis in Bladder Cancer.

International journal of genomics·2025

Related Experiment Video

Updated: Sep 19, 2025

Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs
04:54

Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs

Published on: May 3, 2024

848

Adipose-Derived Stem-Cell-Derived Exosomes Encapsulated Patch for Modulating Inflammation and Promoting Tissue

Yinghua Tao1,2, Tao Liu1,2, Fengya Jing1,2

  • 1State Key Laboratory of Digital Medicine Engineering, Southeast University, Nanjing 210096, China.

ACS Nano
|June 5, 2025
PubMed
Summary

This study presents a Janus G-Avs patch for enhanced tissue repair. The patch delivers adipose-derived stem cell exosomes (ADSC-exo) and prevents adhesions, promoting faster healing and regeneration.

Keywords:
ADSC-exosomesJanus patchcoaxial electrospinninginflammationtissue repair

More Related Videos

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model
08:06

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Published on: August 4, 2017

11.5K
Author Spotlight: Advancing Treatment Approaches with Adipose-Derived Stem Cells
04:53

Author Spotlight: Advancing Treatment Approaches with Adipose-Derived Stem Cells

Published on: January 12, 2024

1.0K

Related Experiment Videos

Last Updated: Sep 19, 2025

Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs
04:54

Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs

Published on: May 3, 2024

848
Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model
08:06

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Published on: August 4, 2017

11.5K
Author Spotlight: Advancing Treatment Approaches with Adipose-Derived Stem Cells
04:53

Author Spotlight: Advancing Treatment Approaches with Adipose-Derived Stem Cells

Published on: January 12, 2024

1.0K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Chronic wound repair remains a significant clinical challenge, with traditional methods showing limitations in recovery speed and biocompatibility.
  • Adipose-derived stem cell exosomes (ADSC-exo) show promise for tissue regeneration due to anti-inflammatory and immunomodulatory properties, but direct stem cell injection has low survival rates.
  • Developing effective delivery systems for ADSC-exo is crucial for maximizing their therapeutic potential in tissue repair.

Purpose of the Study:

  • To develop and evaluate a novel Janus G-Avs patch for enhanced tissue regeneration and prevention of postoperative adhesions.
  • To investigate the capability of the G-Avs patch to deliver ADSC-exo in a controlled manner.
  • To assess the in vivo efficacy of the G-Avs patch in promoting tissue repair and modulating the inflammatory response.

Main Methods:

  • Fabrication of a Janus patch using coaxial electrospinning, with a top layer for ADSC-exo delivery (GelMA/ADSC-exo/HA) and a bottom layer for mechanical support and anti-adhesion (4aPLGA-Glu/PCL).
  • In vivo testing in rat models to evaluate the patch's effects on cell proliferation, angiogenesis, and macrophage polarization.
  • Transcriptomic analysis to elucidate the molecular mechanisms underlying the patch's regenerative effects, focusing on metabolic and immune pathways.

Main Results:

  • The G-Avs patch successfully delivered ADSC-exo, prolonging release and preventing postoperative tissue adhesion.
  • In vivo studies showed enhanced cell proliferation, promoted angiogenesis, and M2 macrophage polarization, indicating reduced inflammation and improved tissue repair.
  • Transcriptomic data revealed that the G-Avs patch upregulates metabolic pathways and downregulates immune-inflammatory pathways, supporting tissue regeneration.

Conclusions:

  • The Janus G-Avs patch is an effective dual-functional material for promoting tissue repair and preventing adhesions.
  • The patch's ability to deliver ADSC-exo and modulate the cellular microenvironment offers a promising therapeutic strategy for chronic wounds.
  • The G-Avs patch demonstrates significant clinical potential for advancing regenerative medicine and wound healing applications.