The ENTAiLS Toolkit: an integrated workflow to perform non-targeted analysis of per- and polyfluoroalkyl substances

Environmental health concerns over per- and polyfluoroalkyl substances (PFAS) have prompted public health laboratories to pursue monitoring studies determining PFAS occurrences. Select laboratories have begun implementing non-targeted analysis (NTA) methods to address the disparity between the few (< 100) PFAS typically measured using targeted analyses and the thousands of known PFAS. While NTA can provide a more comprehensive understanding of PFAS occurrence, significant barriers prevent its widespread adoption in public health laboratories. Notable barriers include a lack of harmonized guidance, standardized methods, and workflows that enable robust NTA application. EPA's Office of Research and Development has been working to overcome these barriers as part of technical collaborations with state environmental and public health laboratories. As a product of these efforts, ORD has developed the ENTAiLS Toolkit, a publicly available compilation of best practices and an open-source data processing workflow for PFAS NTA studies. The ENTAiLS Toolkit offers NTA practitioners comprehensive guidance and integrated tools that can aid the identification and characterization of PFAS in drinking water resources.