COVID-19 detection in bone tissue after a new protocol for decellularize bone with sustained collagen matrix

Area of Science:

• Virology
• Tissue Banking
• Transplant Science

Background:

• The transmissibility of SARS-CoV-2 in organ and tissue donation remains unknown.
• Donor exclusion due to SARS-CoV-2 concerns can lead to critical tissue shortages.
• Balancing viral transmission risk with tissue availability is crucial for patient care.

Purpose of the Study:

• To evaluate the presence of SARS-CoV-2 RNA in bone tissue models post-processing.
• To assess the efficacy of bone processing methods in inactivating SARS-CoV-2.
• To determine the safety of using bone allografts during potential SARS-CoV-2 related shortages.

Main Methods:

• An in vitro study design was employed.
• Bone tissue models were subjected to standard bone processing procedures.
• The presence of SARS-CoV-2 RNA was tested before and after processing.

Main Results:

• SARS-CoV-2 RNA was not detected in bone tissue samples after the processing.
• The bone processing methods demonstrated virucidal activity against SARS-CoV-2.
• No viable virus was found following the established tissue bank procedures.

Conclusions:

• Bone processing effectively inactivates SARS-CoV-2, rendering bone tissue safe for transplantation.
• The use of bone allografts can be considered even during shortages, minimizing transmission risks.
• This study supports the continued availability of bone grafts for patients in need.