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Updated: Sep 19, 2025
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
A DNA Data Storage System Using Electrochemically Active Non-natural Oligonucleotides with Flexible Microfluidic
Jiankai Li1, Ziyan Wang1, Leni Zhong1
1Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, Nanshan District, Shenzhen, Guangdong 518055, P. R. China.
Abstract:
Introducing non-natural oligonucleotides can provide DNA as a data storage medium with higher storage density and novel data storage paradigms. In particular, electrochemically active non-natural oligonucleotides can be detected through electrochemical signals, allowing data storage to retrieve data. Here, we present a DNA-based electrochemical readout data storage system capable of identifying different non-natural, electroactive bases (methylene blue- and ferrocene-modified bases) for DNA. The system utilizes a flexible electrochemical microfluidic chip, where data writing is achieved through DNA hybridization, and the parallel electrochemical signal acquisition enables data reading. Using methylene blue- and ferrocene-modified oligonucleotides as a demonstration, which allows 4 (22) combinations on an electrode for the data storage, we successfully encoded and retrieved a 120-bit text file based on quaternary coding on a flexible electrochemical microfluidic chip. Our system may offer potential applications for electrochemical readout from DNA data storage system.
