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Updated: Sep 19, 2025

Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS
Published on: March 22, 2024
A brief guide to analyzing TurboID-based proximity labeling-mass spectrometry in plants
1Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea.
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Proximity labeling-mass spectrometry has emerged as a powerful biological tool for elucidating the intracellular protein interactome of target proteins. Here, we introduce experimental strategies and methodologies for understanding complex protein-protein interactions in plant cells using the recently developed TurboID-based PL approach. We provide a brief overview of the overall procedures, including the preparation of transgenic Arabidopsis plants, biotin treatment, affinity purification, on-bead digestion, liquid chromatography-tandem mass spectrometry, and data analysis.
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