Related Experiment Video
Updated: Sep 19, 2025
Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
Permethrin exposure increases the adipogenic commitment of mesenchymal stem cells by negatively regulating autophagy
Julee Verma1, Neeraj Kumar Satija1
1Systems Toxicology Group, Food, Drug & Chemical, Environment and Systems Toxicology Division, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh 226001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Abstract:
Permethrin (PER) is a synthetic pyrethroid used in agricultural settings and public health. Previously, PER exposure has been linked with increased adipogenesis. The Wnt/β-catenin pathway is also associated with the regulation of adipogenic differentiation of mesenchymal stem cells (MSCs). This study evaluated the effect of acute PER exposure (24 hr) on the Wnt/β-catenin pathway and its impact on adipogenic commitment of MSC. It also assessed the cross-talk of Wnt/β-catenin signaling and autophagy in PER-mediated adipogenesis. Immunoblotting results showed PER inhibited the Wnt/β-catenin signaling. Nile red staining showed decreased adipogenesis upon PER pre-treatment in combination with lithium chloride compared to PER alone. Further, immunoblotting revealed lithium chloride-mediated activation of autophagy in MSCs. Nile red staining showed inhibition of adipogenic differentiation upon activation of the Wnt/β-catenin pathway and autophagy. Overall, the data showed that cross-talk of autophagy and the Wnt/β-catenin pathway negatively regulates adipogenic commitment of MSCs.
