Related Experiment Video
Updated: Jan 18, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Spectroscopic and Computational Analysis of 5PPD-CeO2: Insights from Fluorescence Quenching and FRET Studies
Kalpana Sharma1,2, Varsha V Koppal3, Raviraj Kusanur4
1Autonomous, affiliated to VTU, Belagavi, Karnataka, 590018, India. kalpanaphysics@gmail.com.
Abstract:
This investigation explores the impact of synthesized cerium oxide nanoparticles (CeO2 NPs) on the solvatochromic behaviour and fluorescence resonance energy transfer (FRET) of the compound 5-phenyl-1-pyridine-3-yl-penta-2,4-dien-1-one in various solvents. The dipole moments of this compound have been estimated using theoretical and experimental methods. Furthermore, the analysis of CeO2 NPs concentration on the organic fluorophore was conducted using the Benesi-Hildebrand equation, the findings indicated a substantial transfer of energy between CeO2 NPs and 5PPD.
Related Concept Videos
Atomic Fluorescence Spectroscopy
UV–Vis Spectroscopy: Molecular Electronic Transitions
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Molecular Spectroscopy: Absorption and Emission
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in...

