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Production of Nanofibrillar Patterned Collagen for Tissue Engineering
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Engineering anti-contractile 3D cellular assemblies using micronozzle-generated fragmented collagen microfibers.

Keigo Yamanaka1, Yuri Shimoda1, Rina Nonogaki1

  • 1Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, Chiba, Japan.

Journal of Biomaterials Science. Polymer Edition
|June 6, 2025
PubMed
Summary

Researchers developed a high-throughput method for creating fragmented collagen microfibers (F-CMFs) for tissue engineering. These microfibers enable the fabrication of advanced 3D tissue models, improving oxygen and nutrient supply for cell growth.

Keywords:
Collagenfibroblastmicrofiberskin tissue modeltissue engineering

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Three-dimensional (3D) cell cultures are crucial for tissue engineering, regenerative medicine, and in vitro drug testing.
  • Bioactive polymer substrates offer potential for improving oxygen and nutrient supply in engineered tissues.
  • Current methods for producing cell-sized materials at high throughput are limited.

Purpose of the Study:

  • To develop a facile and versatile strategy for high-throughput production of fragmented collagen microfibers (F-CMFs).
  • To enable precise control over F-CMF morphology through gelation agent composition.
  • To demonstrate the application of F-CMFs in fabricating advanced 3D tissue models.

Main Methods:

  • Micronozzle-assisted extrusion combined with stirring-induced shear forces for F-CMF production.
  • Controlled composition of type-I collagen gelation agent (polyanion and thickener concentrations).
  • Fabrication of dermal and multilayered human skin tissue models.

Main Results:

  • High-throughput production of fragmented collagen microfibers (F-CMFs) with tunable morphology.
  • F-CMFs effectively suppressed cell-driven tissue contraction in dermal tissue models.
  • Successful formation of multilayered human skin models in microchannel chambers.

Conclusions:

  • The proposed method offers a novel approach for producing F-CMFs for diverse tissue engineering applications.
  • This technique allows precise control over tissue shape and enhances cell-matrix interactions.
  • The developed F-CMFs are promising for creating advanced 3D tissue models with improved cellular functions.