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A reversible decrease in electrical coupling of cultured mouse glial cells induced by superfusion from a

H Kettenmann

    Neuroscience Letters
    |August 16, 1985
    PubMed
    Summary
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    Superfusion from micropipettes can reversibly increase oligodendrocyte input resistance and decrease electrical coupling between cells. This finding suggests careful evaluation of studies using pressure ejection from microelectrodes is necessary.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Oligodendrocyte Research

    Background:

    • Oligodendrocytes are crucial glial cells in the central nervous system, responsible for myelin sheath formation.
    • Understanding cell-cell communication and membrane properties is vital for studying oligodendrocyte function and dysfunction.
    • Micropipette application of substances is a common technique in cellular studies.

    Purpose of the Study:

    • To investigate the effects of superfusion from micropipettes on cultured mouse oligodendrocytes.
    • To determine if superfusion impacts oligodendrocyte input resistance and electrical coupling.

    Main Methods:

    • Cultured mouse oligodendrocytes were superfused using pressure application from a micropipette.
    • Input resistance was measured using two separate electrodes during superfusion.

    Related Experiment Videos

  • Electrical coupling between pairs of oligodendrocytes was assessed under superfusion conditions.

    • Main Results:

    • Superfusion reversibly increased input resistance by 90% in 26 tested oligodendrocytes.
    • Input resistance remained unaffected in 39 other cells.
    • Electrical coupling between oligodendrocyte pairs decreased reversibly during superfusion.

    Conclusions:

    • Fluid flow from micropipettes can induce cell uncoupling in oligodendrocytes.
    • Experimental findings from studies using pressure ejection from microelectrodes should be interpreted with caution.
    • The superfusion technique itself may influence oligodendrocyte electrophysiological properties and cell-cell communication.