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  1. Home
  2. When Is Microbial Cross-feeding Evolutionarily Stable?
  1. Home
  2. When Is Microbial Cross-feeding Evolutionarily Stable?

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When is microbial cross-feeding evolutionarily stable?

Jamie Alcira Lopez1,2,3, Bo Liu4,5, Zhiyuan Li6,7

  • 1Lewis-Sigler Institute for Integrative Genomics, Princeton University, New Jersey 08544, USA.

Biorxiv : the Preprint Server for Biology
|June 6, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Waste-product cross-feeding, where microbes share metabolic byproducts, is common but poorly understood. This study reveals generalized intracellular metabolite toxicity as a key mechanism enabling stable cross-feeding microbial consortia.

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Area of Science:

  • Microbial Ecology
  • Evolutionary Biology
  • Biochemistry

Background:

  • Cross-feeding, the exchange of metabolites between organisms, is prevalent in microbial communities.
  • Waste-product cross-feeding, a unidirectional interaction, is a common form where one microbe's waste supports another's growth.
  • The evolutionary persistence of waste-product cross-feeding, despite potential for single-organism efficiency, remains unclear.

Purpose of the Study:

  • To investigate the evolutionary stability of waste-product cross-feeding.
  • To identify mechanisms that explain why microbial consortia engage in cross-feeding.
  • To understand the conditions promoting the stability of cross-feeding interactions.

Main Methods:

  • Analysis of cross-feeding evolution using a minimal model of microbial metabolism.
  • Thermodynamically correct formulation of multi-step energy extraction.
  • Investigation of models with complex growth functions to identify stability mechanisms.
  • Main Results:

    • Cross-feeding was not evolutionarily stable in the minimal metabolic model.
    • Generalized intracellular metabolite toxicity was identified as a novel mechanism for cross-feeding stability.
    • Nonlinear scaling of growth penalties with intracellular metabolite levels promotes cross-feeding.

    Conclusions:

    • Generalized intracellular metabolite toxicity can stabilize waste-product cross-feeding consortia.
    • Consortia can divide toxic metabolite burdens for greater collective efficiency.
    • Cross-feeding stability mechanisms can lead to complex population dynamics, including biomass discontinuities.