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Related Concept Videos

RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Chromatin Structure Regulates pre-mRNA Processing02:41

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Negative Regulator Molecules01:23

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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Pre-mRNA Processing: RNA Splicing01:36

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Related Experiment Video

Updated: Sep 19, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

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The Splicing Factor PTBP1 interacts with RUNX1 and is Required for Leukemia Cell Survival.

Arjun Dhir, Alexander Ethell, Riley Watkins

    Biorxiv : the Preprint Server for Biology
    |June 6, 2025
    PubMed
    Summary
    This summary is machine-generated.

    Polypyrimidine Tract Binding Protein 1 (PTBP1) interacts with Runt-related Transcription Factor 1 (RUNX1) in leukemia cells. This interaction is crucial for metabolic gene expression, impacting leukemia cell survival.

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    Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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    Area of Science:

    • Hematopoiesis and Leukemia Research
    • Molecular Biology
    • Cancer Genomics

    Background:

    • Runt-related Transcription Factor 1 (RUNX1) is vital for blood formation and frequently mutated in leukemia.
    • Histone Deacetylase 1 (HDAC1) partners with RUNX1 and plays a role in active transcription.
    • A non-histone function of HDAC1 in regulating RUNX1 complex components is suggested.

    Purpose of the Study:

    • To identify novel RUNX1 interacting partners in leukemia.
    • To investigate the role of these partners in gene regulation and RNA splicing.
    • To elucidate the functional significance of the RUNX1-PTBP1 interaction in leukemia.

    Main Methods:

    • Proteomics, genomics, and long-read transcriptomics were employed.
    • Chromatin profiling was used to map factor occupancy.
    • Functional studies involved assessing the impact of PTBP1 loss in AML cells.

    Main Results:

    • Polypyrimidine Tract Binding Protein 1 (PTBP1) was identified as a RUNX1 interactor, dependent on HDAC1.
    • RUNX1 and PTBP1 extensively co-occupied gene promoters, particularly for actively transcribed genes.
    • Loss of PTBP1 caused widespread RNA splicing alterations and reduced expression of metabolic genes.

    Conclusions:

    • PTBP1 binds RUNX1 in an HDAC1-dependent manner, co-localizing at target gene promoters.
    • PTBP1 loss in leukemia cells impairs metabolic gene expression, leading to reduced growth, glycolysis, and cell death.