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Related Experiment Video

Updated: Sep 19, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Super-Resolution Confocal Microscopy Enabled by Fluorescence Intensity Ratio Encoding and Difference.

Junle Qu¹, Xuegang Zhu¹, Dongyi Liu¹

¹State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.

|June 6, 2025

Summary

This summary is machine-generated.

We developed fluorescence intensity ratio encoding and difference (FIRED) microscopy, a super-resolution technique. FIRED microscopy significantly enhances resolution and contrast in fluorescence imaging for biological and material sciences.

Keywords:

confocal microscopy diffraction limit fluorescence encoding live-cell imaging super-resolution microscopy

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Area of Science:

Optics and Photonics
Biomedical Imaging
Materials Science

Background:

Conventional confocal microscopy is limited by the optical diffraction barrier.
Achieving higher resolution in fluorescence microscopy is crucial for detailed biological and material analysis.

Purpose of the Study:

To introduce a novel far-field super-resolution microscopy technique called FIRED microscopy.
To overcome the diffraction limit in fluorescence imaging and improve resolution and contrast.

Main Methods:

FIRED microscopy dynamically modulates the excitation spot in an optimized confocal microscope.
It uses fluorescence intensity ratios from two excitation paths to create a weight matrix.
This matrix generates a super-resolution FIRE image, which is then processed to produce the final FIRED image.

Main Results:

FIRED microscopy achieves up to a 4-fold resolution improvement over conventional confocal microscopy.
A lateral resolution of 71.2 nm was experimentally validated.
The method demonstrates an enhanced signal-to-background ratio and artifact suppression.

Conclusions:

FIRED microscopy is an innovative far-field super-resolution method.
It effectively surpasses the optical diffraction barrier in fluorescence microscopy.
FIRED is a robust tool for advancing biological and materials science applications.