Related Experiment Video
Updated: Jun 15, 2025
Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
Published on: May 26, 2023
High-Resolution, Motion-Corrected, Volume-Fused OCT for Investigating Longitudinal Changes in Subretinal Drusenoid
Jungeun Won1, Antonio Yaghy2, Stefan B Ploner3
1Department of Electrical Engineering and Computer Science, and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA.
Purpose:
To demonstrate high-resolution, motion-corrected, volume-fused optical coherence tomography (OCT) for assessing longitudinal changes in macular dot form subretinal drusenoid deposits (SDDs).
Methods:
Six consecutive isotropic volume raster scans over 6 × 6 mm (500 × 500 A-scans) were acquired using a high-resolution (2.7 µm axial resolution) spectral domain OCT prototype instrument. OCT volumes were computationally motion-corrected and fused. The distribution and longitudinal changes in dot SDDs were evaluated using en face OCT in a 50-µm-thick slab, from 27 µm above Bruch's membrane.
Results:
Computational motion correction and volume fusion methods improve visibility of small en face features and compensate for motion artifacts to facilitate longitudinal assessment. In total, 326 SDDs were identified in a representative series of four eyes from four patients with intermediate age-related macular degeneration (AMD) and assessed with a 3- to 12-month follow-up. Of the SDDs, 85.3% remained stable over the follow-up, while 9.8% regressed, 3.4% fused, and 1.5% new SDDs appeared.
Conclusions:
Computational motion correction and volume fusion combined with high-resolution OCT B-scans and en face slabs facilitate visualization and longitudinal tracking of focal pathologies, such as SDDs.
Translational Relevance:
The methods presented have the potential to improve OCT analysis of focal features, such as quantification of SDDs and other AMD biomarkers.
