Formulation of a stable p-acetylaminohippurate infusion fluid and determination of the serum concentrations

An infusion fluid with p-acetylaminohippurate (PACAH) was formulated and a method for analysis of PACAH in serum, urine and infusion fluid based on reversed phase HPLC was developed. No significant degradation could be observed after steam sterilization for twenty minutes at 120 degrees C. From the results of an investigation of the degradation rate at elevated temperatures a negligible loss of content during storage for three years at 20 degrees C could be predicted. Steady state serum concentrations of PACAH after constant infusion were determined.