Color mapping of multitarget protein clusters

Visualizing subcellular distributions of proteins and assessing their colocalization patterns are central to understanding the organization and interactions of molecular assemblies. In this issue of Structure, Kiuchi et al.¹ introduce protein cluster coloring to map complex association patterns among eight endogenous proteins at the periphery of the clathrin-coated structure, revealing their multilayered complex associations upon epidermal growth factor stimulation.