Laccase immobilized on electrospun polyurethane/regenerated cellulose nanofiber membranes for efficient...

Laccase has been the focus of extensive research in the field of pollutant degradation. However, its practical application is currently severely hampered by issues such as poor stability, proneness to inactivation, and recycling challenges. In this study, numerous laccase molecules were efficiently loaded onto the electrospun polyurethane/regenerated cellulose nanofiber membrane via metal ion coordination technique. A comprehensive analysis of the optimal fixation conditions and stability of laccase was performed. Furthermore, the catalytic mechanism of the nanofiber membrane immobilized enzyme toward P-Chlorophenol was meticulously investigated. Results reveal that the metal ion coordination method can efficiently immobilize laccase. Among them, the immobilization amount of laccase immobilized on the nanofiber membrane via Cu (II) coordination peaks, reaching 136 mg/g. The activity of the immobilized enzyme remained higher than 80 % after 20 days of storage. When the concentration of P-chlorophenol is 10 mg/L, the nanofiber membrane immobilized enzyme shows an 81.49 % degradation efficiency, following second-order reaction kinetics. The nanofiber membrane immobilized enzyme maintains a degradation rate of over 58 % after multiple reuse cycles, demonstrating its excellent reusability. Research demonstrates that the nanofiber membrane immobilized enzyme has great application potential in water pollution treatment.