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Studies on globin chain synthesis during hamster development.

T Boussios, J F Bertles

    Progress in Clinical and Biological Research
    |January 1, 1985
    PubMed
    Summary

    Yolk-sac-derived erythroid cells in hamster embryos exhibit pre-programmed globin gene expression. These primitive cells maintain their developmental program even when cultured, offering insights into globin gene ontogeny.

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    The globin gene expression program in the hamster embryo.

    Experimental hematology·1988

    Area of Science:

    • Developmental Biology
    • Hematopoiesis
    • Molecular Biology

    Background:

    • Yolk-sac-derived erythroid cells are the primary circulating erythroid cells in early hamster embryos.
    • These cells actively synthesize hemoglobin and are the sole erythroid tissue until fetal liver development.
    • Their distinct size allows for separation from later-developing erythrocytes.

    Purpose of the Study:

    • To investigate the globin gene expression program in primitive erythroid cells.
    • To determine if this program is pre-programmed and can function independently of the embryonic environment.
    • To explore the potential of cultured erythroid cells for studying globin gene ontogeny.

    Main Methods:

    • Isolation and culture of yolk-sac-derived erythroid cells from hamster embryos.
    • Electrophoretic separation of embryonic and adult globin chains.
    • Comparison of globin chain synthesis in vivo and in vitro.

    Main Results:

    • Hamster embryonic and adult globin chains separate distinctly via electrophoresis.
    • Globin chain ontogeny in circulating blood is nearly complete by birth.
    • Cultured yolk-sac erythroid cells synthesized globin chains consistent with in vivo developmental changes.

    Conclusions:

    • The ontogenic switch of globin gene expression in these primitive erythroid cells is pre-programmed.
    • This program functions effectively in culture, independent of the embryonic environment.
    • Studying these cells in culture facilitates research into the mechanisms of globin gene ontogeny.

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