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Updated: Sep 19, 2025

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
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Negative feedback equalizes polarity sites in a multi-budding yeast.

Alex W Crocker1, Claudia A Petrucco2, Kaiyun Guan3

  • 1Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, 120 Mason Farm Rd., Chapel Hill, NC 27599, USA; Duke University, Department of Cell Biology, 308 Research Drive, Durham, NC 27705, USA.

Current Biology : CB
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PubMed
Summary

Aureobasidium pullulans yeast forms multiple buds by independently regulating cell polarity sites. This contrasts with single-budding yeast and reveals how conserved signaling networks create diverse fungal morphogenesis.

Keywords:
Cdc42Pak1extremotolerant yeastsmulti-buddingpolarity

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Area of Science:

  • Cell Biology
  • Mycology
  • Developmental Biology

Background:

  • Fungal and animal morphogenesis rely on small GTPases like Cdc42 and Rac for cell polarization.
  • Budding yeast Saccharomyces cerevisiae typically forms a single bud due to competition between polarity sites.

Purpose of the Study:

  • To investigate the mechanism of cell polarity and multiple budding in Aureobasidium pullulans.
  • To understand how conserved signaling networks are modulated for distinct morphogenic programs.

Main Methods:

  • Observation of coexisting polarity sites and independent oscillations of polarity machinery components.
  • Identification of Pak1 as a key protein regulating negative feedback in the polarity circuit.
  • Assessing the requirement of Rac1 and Cdc42 for Pak1 localization.

Main Results:

  • Aureobasidium pullulans establishes multiple coexisting polarity sites, leading to multiple buds per cell cycle.
  • Polarity components oscillate independently across sites, indicating a lack of global coupling.
  • Negative feedback, crucial for multiple polarity sites and oscillations, depends on Pak1, which requires Rac1 but not Cdc42.

Conclusions:

  • Conserved cell polarity signaling networks can be adapted for diverse morphogenic outcomes, such as multiple budding in fungi.
  • Pak1-mediated negative feedback is essential for the distinct budding pattern observed in Aureobasidium pullulans.
  • Independent regulation of polarity sites allows for complex developmental programs within conserved molecular frameworks.