Probiotic bacteria from asymptomatic necrotic tooth can regulate the microbiome homeostasis

Area of Science:

• Microbiology
• Endodontics
• Biotechnology

Background:

• Chronic asymptomatic necrotic root canals harbor complex microbial communities.
• Maintaining root canal microbiome homeostasis is crucial for treatment success.
• The role of specific bacteria and their products in this environment is not fully understood.

Purpose of the Study:

• To identify and isolate dominant bacteria from chronic asymptomatic necrotic root canals.
• To investigate the in vitro postbiotic potential of isolated bacteria to maintain root canal microbiome homeostasis.
• To evaluate the antibiofilm effects of bacterial cell-free supernatant (CFS) against pathogenic bacteria.

Main Methods:

• Metagenomic analysis and single colony isolation for bacterial identification.
• Cultivation of Lacticaseibacillus paracasei and preparation of cell-free supernatant (CFS).
• In vitro biofilm analysis against Streptococcus mutans and Enterococcus faecalis using crystal violet staining and microscopy (SEM).

Main Results:

• The dominant isolated bacterium was identified as Lacticaseibacillus paracasei.
• L. paracasei produced biofilm mass comparable to Enterococcus faecalis.
• The CFS of L. paracasei demonstrated significant disruptive effects on S. mutans and E. faecalis biofilms.

Conclusions:

• Lacticaseibacillus paracasei isolated from necrotic root canals produces postbiotic products.
• These postbiotics effectively disrupt the biofilm integrity of key oral pathogens, S. mutans and E. faecalis.
• This suggests a potential therapeutic role for L. paracasei-derived postbiotics in managing root canal infections.