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Coumarin bialdehyde-based fluorescent probe for the detection of hydrazine in living cells, soil samples and its
Lin Shan1, Xuewei Li1, Zhe Yu1
1Key Laboratory of Photochemical Conversion and Optoelectronic Materials & CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Abstract:
As an essential fine chemical material, the prevalent application of hydrazine (N2H4) inevitably endangers environmental safety and biological health. Herein, we propose a coumarin derivative (Cou-N-2CHO) with a unique bialdehyde structure for the detection of hydrazine. Both aldehyde groups of Cou-N-2CHO could be nucleophilically attacked by hydrazine, resulting in a new adduct with significant fluorescence enhancement (22-fold). Possible responsive mechanism has been verified by NMR and ESI-MS analyses. Cou-N-2CHO was sensitive to hydrazine with a limit of detection (LOD) as low as 1.02 μM. Moreover, Cou-N-2CHO showed good specificity and excellent immunity to interference in response to hydrazine. Apart from successfully enabling cell imaging of hydrazine, Cou-N-2CHO can also be applied in tests of paper strips and soil analysis. More meaningfully, this work opens up more possibilities to detect hydrazine in multiple environmental samples.
