Expression of the immune gene related to energy metabolism (NDRG1) in Wilms' tumor and its correlation with prognosis
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View abstract on PubMed
Summary
This summary is machine-generated.NDRG1 is downregulated in Wilms tumor (WT), correlating with poor prognosis and inhibiting tumor growth. This suggests NDRG1 is a potential prognostic biomarker linked to immune cell infiltration in WT.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Research
Background
- Wilms tumor (WT) is a pediatric kidney cancer with varying prognoses.
- Understanding the molecular mechanisms driving WT progression is crucial for improving patient outcomes.
Purpose Of The Study
- To investigate the expression of NDRG1 in Wilms tumor.
- To analyze the correlation between NDRG1 expression and WT prognosis.
- To elucidate the role and potential mechanisms of NDRG1 in WT development and progression.
Main Methods
- NDRG1 expression was analyzed in WT cohorts (TARGET, GSE138869).
- Prognostic value was assessed using Cox regression, decision curve analysis, and nomograms.
- Immune infiltration was evaluated using ESTIMATE and CIBERSORT.
- Downregulation factors were identified via cBioPortal and StarBase.
- In vitro functional assays (CCK8, Transwell) were performed.
Main Results
- NDRG1 was downregulated in WT, particularly in patients with unfavorable prognosis and advanced stages.
- Low NDRG1 expression correlated with a poorer immune microenvironment and reduced immune cell infiltration.
- Functional enrichment analysis linked NDRG1 to DNA binding, tubulin binding, and glycosaminoglycan binding pathways.
- Gene Set Enrichment Analysis (GSEA) associated NDRG1 with pathways like the Nod-like receptor signaling pathway and DNA replication.
- Overexpression of NDRG1 inhibited WT cell proliferation and migration in vitro.
Conclusions
- NDRG1 is downregulated in Wilms tumor and associated with adverse prognostic factors.
- NDRG1 expression is linked to the tumor immune microenvironment and immune cell infiltration.
- NDRG1 may serve as a promising prognostic biomarker for Wilms tumor.
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