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Male prepubertal fertility preservation: a review

Campbell Vogt1, Neha R Malhotra2

  • 1Department of Urology, Icahn School of Medicine at Mount Sinai, New York, New York.

Fertility and Sterility
|June 8, 2025
PubMed

Insights

Fertility preservation for prepubertal boys is crucial due to gonadotoxic cancer therapies. Testicular cryopreservation is common, but maturing harvested cells remains a challenge for future fertility.

Area of Science:

  • Reproductive Medicine
  • Pediatric Oncology
  • Cell Biology

Background:

  • Cancer treatments like chemotherapy, radiation, and hormonal therapies can impair future fertility.
  • Pediatric patients undergoing gonadotoxic therapies face risks to their reproductive potential.
  • Prepubertal boys cannot use traditional sperm cryopreservation as they do not produce mature sperm.

Purpose of the Study:

  • To review current fertility preservation methods for prepubertal males.
  • To highlight testicular cryopreservation as a primary method.
  • To identify research gaps in advancing fertility preservation for this population.

Main Methods:

  • Literature review of current fertility preservation techniques.
  • Focus on testicular cryopreservation in prepubertal males.
  • Analysis of challenges and future research directions.

Main Results:

  • Testicular cryopreservation is the most common method for prepubertal male fertility preservation.
  • Animal models show promise, but clinical application in humans is limited.
  • Maturation of harvested spermatogonia remains a significant hurdle for clinical use.

Conclusions:

  • Further research is needed to overcome the limitations of current prepubertal male fertility preservation methods.
  • Developing techniques to mature spermatogonia is critical for clinical success.
  • Advancing fertility preservation is essential for pediatric cancer survivors.
Keywords:
Cryopreservationchildfertility preservation

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