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Liquid Y2H-Seq, a Rapid and Data-Rich Alternative to Conventional Yeast Two-Hybrid Screening
Yanping Qi1, Chunlei Zhang1, Shan Yuan1
1Ministry of Agriculture and Rural Affairs Key Lab of Soybean Biology, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
None:
Highly laborious plating of cultures onto solid media is inevitable in conventional yeast two-hybrid systems. We have successfully developed Liquid Y2H-Seq, a method that replaces solid media with liquid media for culturing and displaces visual inspection of colonies with sequencing. Soybean (Glycine max) is a typical photoperiod-sensitive crop, meaning that a specific duration of light regime has a huge impact on soybean flowering and production. We obtained a lot of putative interactions for soybean flowering genes. Finally, these reports introduce a labor-reducing and time-saving method for identifying protein-protein interactions, based on simple modifications to a ubiquitous protocol in life science research.
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