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Effect of overexpression of GRN on the proliferation and osteogenic capacity of human periodontal cells

  • 0School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Experimental and Therapeutic Medicine +

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June 9, 2025

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June 9, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Overexpressing the granulin precursor gene (GRN) in human periodontal ligament cells (hPDLCs) significantly boosted cell proliferation and osteogenic capacity. This research supports periodontal tissue regeneration strategies.

Area Of Science

  • Cell Biology
  • Regenerative Medicine
  • Genetics

Background

  • Human periodontal ligament cells (hPDLCs) are crucial for periodontal tissue regeneration.
  • The granulin precursor gene (GRN) role in hPDLCs requires further investigation.

Purpose Of The Study

  • To establish a stable hPDLC cell line overexpressing GRN.
  • To investigate the effects of GRN on hPDLC proliferation and osteogenic differentiation.

Main Methods

  • Lentiviral mediation was used to construct a pLV-GRN plasmid for stable GRN overexpression in hPDLCs.
  • Cell proliferation was assessed using the MTT assay.
  • Osteogenic capacity was evaluated through Alizarin red staining, alkaline phosphatase (ALP) activity assays, and RT-qPCR/Western blotting for osteogenic gene expression (ALP, Runx2, OPN).

Main Results

  • A stable hPDLC cell line overexpressing GRN was successfully established.
  • GRN overexpression significantly enhanced hPDLC proliferation compared to control groups (P<0.05).
  • Osteogenic capacity, including ALP activity and expression of osteogenic genes (ALP, Runx2, OPN), was significantly increased in GRN-overexpressing cells (P<0.01 for Alizarin red/ALP, P<0.05 for gene expression).

Conclusions

  • Overexpression of GRN significantly promotes the proliferation and osteogenic differentiation of hPDLCs.
  • These findings provide a potential therapeutic target and experimental basis for periodontal tissue regeneration.
  • GRN holds promise for enhancing regenerative capabilities in periodontal defects.

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