Related Experiment Video

Updated: Jun 12, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

Circular RNA hsa_circRNA_101996 modulates gastric cancer cell proliferation and apoptosis through the miR-577/HMGN5

Xiao-Lei Wang1, Lin Zhang2, Qing Shang3

  • 1Department of General Surgery, Xinxiang Central Hospital, Xinxiang 453000, Henan Province, China. wangxl305@163.com.

World Journal of Gastrointestinal Oncology
|June 9, 2025
PubMed
Abstract

Insights

Circular RNAs (circRNAs) promote gastric cancer by upregulating HMGN5 via sponging miR-577. This study identifies hsa_circRNA_101996 as a potential therapeutic target for gastric cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are key regulators in cancer development.
  • While many circRNAs are linked to gastric cancer, the function of hsa_circRNA_101996 is not well understood.
  • This study investigates hsa_circRNA_101996's role in gastric cancer.

Purpose of the Study:

  • To determine the role of hsa_circRNA_101996 in gastric cancer cell proliferation and apoptosis.
  • To elucidate the mechanism involving microRNA-577 (miR-577) and high mobility group nucleosome binding domain 5 (HMGN5).

Main Methods:

  • Analysis of 41 paired gastric cancer tissues and adjacent non-cancerous tissues.
  • Utilized bioinformatics datasets (GSE83521, GSE89143) for differential circRNA expression analysis.
  • Performed in vitro experiments (MTT, colony formation, Western blot, dual-luciferase reporter assays) and in vivo tumorigenesis studies in nude mice.

Main Results:

  • Hsa_circRNA_101996 was significantly upregulated in gastric cancer tissues and cell lines.
  • Validated interactions between hsa_circRNA_101996, miR-577, and HMGN5.
  • Overexpression of hsa_circRNA_101996 increased proliferation and decreased apoptosis in vitro, while miR-577 mimics had opposite effects. In vivo studies confirmed increased tumor volume and HMGN5 expression.

Conclusions:

  • Hsa_circRNA_101996 promotes gastric cancer progression by acting as a sponge for miR-577, leading to increased HMGN5 expression.
  • This circRNA-miRNA-mRNA axis represents a potential novel therapeutic target for gastric cancer.
Keywords:
ApoptosisCircRNA_101996Gastric cancerHigh mobility group nucleosome binding domain 5MicroRNA-577Proliferation

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