Rutin Protects Myocardial Ischemia-Reperfusion Injury via the NF-κB/NLRP3/Pyroptosis Pathway

Area of Science:

• Cardiology
• Biochemistry
• Pharmacology

Background:

• Myocardial ischemia-reperfusion (I/R) injury is a significant clinical challenge.
• Understanding the molecular mechanisms underlying I/R injury is crucial for developing effective treatments.

Purpose of the Study:

• To investigate the protective effects and underlying mechanisms of rutin on myocardial I/R injury.
• To explore rutin's role in regulating pyroptosis and inflammation.

Main Methods:

• Established a myocardial I/R injury model in vivo and in vitro.
• Assessed myocardial infarct size, apoptosis, cell viability, and inflammatory markers.
• Utilized techniques including triphenyltetrazolium chloride staining, TUNEL assay, Western blot, ELISA, and CCK-8 assay.
• Investigated the involvement of the NF-κB/NLRP3/pyroptosis pathway.

Main Results:

• Rutin significantly reduced myocardial infarct area and apoptosis in vivo.
• Rutin treatment decreased levels of cardiac injury markers (CK-MB, cTnT/I) and malondialdehyde.
• In vitro, rutin enhanced cell viability and reduced lactate dehydrogenase (LDH) and reactive oxygen species (ROS) levels.
• Rutin downregulated key pyroptosis-related proteins (NF-κB, NLRP3, cleaved caspase-1, cleaved gasdermin D) and inflammatory factors (IL-1β, TNF-α, IL-18).

Conclusions:

• Rutin exerts protective effects against myocardial I/R injury.
• The protective mechanism involves the inhibition of the NF-κB/NLRP3/pyroptosis pathway.
• Rutin represents a promising therapeutic agent for managing myocardial I/R injury.