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Updated: Feb 26, 2026
08:17
Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure
Published on: August 25, 2017
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Ozone Generation Method Impacts Lung Toxicity and Oxidant Signaling
Atefeh Razazan1,2, Md Habibul Hasan Mazumder1,2, William Travis Goldsmith1,2
1Department of Physiology, Pharmacology, and Toxicology.
Summary
Ozone (O3) generation method impacts lung injury. Using filtered air for O3 production, versus pure oxygen, increased nitrogen oxides (NOx) and lung inflammation, especially when combined with carbon black particles.
Area of Science:
- Environmental Health
- Toxicology
- Respiratory Medicine
Background:
- Ozone (O3) is a pollutant expected to increase due to climate change.
- O3 generation methods can produce varying amounts of nitrogen oxides (NOx) as byproducts.
- Inconsistent monitoring of NOx during O3 generation may explain differing experimental outcomes.
Purpose of the Study:
- To compare the effects of O3 generated from filtered air versus pure oxygen on NOx production and lung injury.
- To investigate the impact of O3 generation source on mixed exposures with O3 and carbon black (CB).
Main Methods:
- Comparison of O3 generation from filtered air (FA-ozone) vs. pure oxygen (Oxy-ozone).
- Assessment of NOx generation and lung injury markers (inflammation, protein nitrotyrosine, eosinophil recruitment).
- Evaluation of mixed exposures: FA-ozone-CB vs. Oxy-ozone-CB.
Main Results:
- Filtered air-generated ozone (FA-ozone) caused increased lung inflammation and injury compared to pure oxygen-generated ozone (Oxy-ozone).
- FA-ozone-CB exposure led to higher NOx formation and protein nitrotyrosine in lungs versus Oxy-ozone-CB.
- Eosinophil recruitment was observed in the FA-ozone-CB group but not in the Oxy-ozone-CB group.
Conclusions:
- The source of oxygen used for O3 generation significantly impacts experimental outcomes, including lung injury.
- Consistent measurement and reporting of nitrogen species during O3 exposure studies are crucial.
- Understanding O3 generation byproducts is essential for accurate exposure assessment and toxicological studies.
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