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Prevalence of Rotavirus among Children in Baghdad, Iraq, detected by molecular methods

Maryam Kareem Ali1, Jaafar Sataar Shia2

  • 1Department of Microbiology, College of Medicine, University of Baghdad, Iraq.

Annali Di Igiene : Medicina Preventiva E Di Comunita
|June 9, 2025
PubMed

Insights

Rotavirus infection affects many children under five in Baghdad, Iraq, with an 80.8% prevalence found. Molecular techniques confirmed rotavirus strains, highlighting the need for vaccination and surveillance.

Area of Science:

  • Pediatrics
  • Infectious Diseases
  • Molecular Epidemiology

Background:

  • Rotavirus is a primary cause of severe gastroenteritis and dehydration in young children globally.
  • Understanding rotavirus prevalence is crucial for public health interventions.

Purpose of the Study:

  • To determine the prevalence of rotavirus infection in children under five years old in Baghdad, Iraq.
  • To assess the utility of molecular techniques for rotavirus detection.

Main Methods:

  • Collection of 120 stool specimens from children with diarrhea between November 2022 and August 2023.
  • Utilized immunochromatographic test and reverse transcriptase-polymerase chain reaction (RT-PCR) for rotavirus detection.
  • RT-PCR was used to detect and characterize the VP7 gene.

Main Results:

  • An overall rotavirus prevalence of 80.8% was observed in the study population.
  • Rotavirus infection was more prevalent in males (63.92%) and children aged 13-24 months (50.5%).
  • RT-PCR identified 97 cases (80.8%) positive for the VP7 gene.

Conclusions:

  • Rotavirus poses a significant health burden on young children in Baghdad.
  • RT-PCR is an effective method for detecting rotavirus strains.
  • Findings support continued rotavirus surveillance and vaccination programs in Iraq.
Abstract

Keywords:
Rotavirus; children; molecular diagnosis; VP7 gene; Baghdad

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