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Related Experiment Video

Updated: Sep 19, 2025

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Cytotransducers for Visualization of Spatiotemporal Intercellular Communication.

Niko Kimura1,2, Yoko Yamanishi3, Shinya Sakuma3

  • 1Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Small (Weinheim an Der Bergstrasse, Germany)
|June 9, 2025
PubMed
Summary

Researchers developed novel cytotransducers for visualizing cell-to-cell communication. These nano-artifacts enable spatiotemporally controlled imaging of intracellular pH, offering new insights into multicellular interactions.

Keywords:
cellular uptakesfluorescent nanodiamondsintercellular communicationslipid nanoparticlesmicrofluidics

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Intercellular communication is vital for multicellular organisms.
  • Current visualization methods using nano-artifacts are limited by diffusion-based transport.
  • Spatiotemporally controlled visualization of cell interactions remains a challenge.

Purpose of the Study:

  • To conceptualize and develop cytotransducers for spatiotemporally controlled visualization of intercellular communication.
  • To enable the conversion of veiled intra/intercellular information into visible signals.
  • To demonstrate cross-scale visualization of cell interactions.

Main Methods:

  • A 110-nm cytotransducer was designed, comprising a lipid layer and a fluorescent nanodiamond.
  • The lipid layer facilitated neutrophil uptake.
  • Fluorescein isothiocyanate labeling enabled visualization of intracellular pH.

Main Results:

  • The cytotransducer was successfully used to visualize intercellular communication between neutrophils and macrophages, aged cells, and mesenchymal stem cells.
  • Intracellular pH maps were generated before and during cell interactions.
  • Spatiotemporally controlled visualization was achieved via localized cytotransducer release during NETosis.

Conclusions:

  • The developed cytotransducers enable precise, spatiotemporally controlled visualization of intercellular communication.
  • This technology allows simultaneous visualization of intra/intercellular information.
  • The findings offer a new approach for studying complex multicellular systems.