Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

Mutations

Mutations

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural basis of lipid-dependent allosteric gating mechanisms for PC1-PC2 ion channel.

Nature communications·2026

Same author

A neuron type-specific microexon in Ank3/ankyrin-G modulates calcium activity and neuronal excitability.

Nature communications·2026

Same author

A Ciliary Phosphoinositide Pathway Regulates the Dosage of Polycystins in Primary Cilia.

Journal of the American Society of Nephrology : JASN·2026

Same author

Advances in Human Metapneumovirus Research: Clinical Impact, Diagnostic Innovations, and Therapeutic Challenges.

Pathogens (Basel, Switzerland)·2025

Same author

Synchrony of neuromodulatory systems during NREM sleep.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Functional divergence of Capicua isoforms explains differential tissue vulnerability in neurological disease.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: Jun 13, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Alternative Splicing and CaV-Associated Channelopathies.

Willy Munyao¹, Md Mostafizur Rahman¹, Samuel A Sabzanov¹

¹Laboratory of RNA Biology and Molecular Neuroscience, Department of Biological Sciences, St. John's University, Queens, New York, USA.

Wiley Interdisciplinary Reviews. RNA

|June 10, 2025

Summary

Voltage-gated calcium channels (VGCCs) regulate physiology, but their dysfunction causes disease. Targeting alternative splicing of VGCCs offers a new therapeutic strategy for channelopathies and neurodevelopmental disorders.

Keywords:

alternative splicing calcium channel disease ion channel therapy

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Related Experiment Videos

Last Updated: Jun 13, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular biology
Neuroscience
Ion channel research

Background:

Voltage-gated calcium channels (VGCCs) are crucial for physiological processes.
Dysfunctional VGCCs are linked to neurological, cardiac, psychiatric, endocrine, oncogenic, and muscular disorders.
Alternative splicing of VGCC genes generates diverse cell-specific variants.

Purpose of the Study:

To highlight the role of alternative splicing in VGCC function and pathology.
To explore the therapeutic potential of targeting VGCC alternative splicing.

Main Methods:

Analysis of VGCC gene expression and alternative splicing patterns.
Investigating the impact of splicing factors on CaV variant generation.
Reviewing genetic mutations affecting splicing in channelopathies.

Main Results:

All 10 VGCC α1-subunit genes undergo alternative splicing, creating diverse CaV variants.
Splicing factors tightly regulate the cell-specific expression of these variants.
Aberrant splicing due to mutations in cis-acting elements or splicing machinery causes channelopathies.

Conclusions:

Alternative splicing is critical for VGCC diversity and function.
Targeting disease-associated splicing events is a promising therapeutic approach for channelopathies and neurodevelopmental disorders.