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Updated: Jun 14, 2025
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
Published on: January 22, 2015
Development, characterization and in vitro evaluation of capecitabine-loaded chitosan nanoparticles
Debarati Ghosh1, Ketousetuo Kuotsu1
1Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.
Objective:
To formulate capecitabine-loaded nanoparticles by the ionotropic gelation method, using low molecular weight (LMW) chitosan polymer and sodium tripolyphosphate (STPP) as the cross-linking agent.
Methods:
Nanoparticles were prepared using the ionotropic gelation method. They were characterized for particle size, polydispersity index (PDI), entrapment efficiency (%), and percentage yield (%) using dynamic light scattering (DLS), UV-Visible spectrophotometry, and other analytical techniques.
Results:
The optimized formulation (CN-4) showed a particle size of 166.5 nm ± 2.3 nm, PDI of 0.346 ± 0.01, entrapment efficiency of 65.34 ± 1.8%, and percentage yield of 90.32 ± 2.1%. The zeta potential of CN-4 was found to be +37.4 mV, confirming its colloidal stability and cationic surface characteristics. An increase in particle size and PDI was observed with higher concentrations of chitosan.
Conclusions:
No appreciable difference in particle size, polydispersity index (PDI) and percentage intensity was observed for CN-4 formulation after storing it for a period of 15 days at 2-4 °C. However, significant changes were observed after 30 days of storage, indicating destabilization and precipitation of the formulation.
