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Enhanced production of ergothioneine in Aspergillus oryzae.

Lihong Wang1,2, Xueqin Tian1,2, Pinghong Xue1,2

  • 1College of Life Science, Jiangxi Science & Technology Normal University, Nanchang, 330013, Jiangxi, China.

Applied Microbiology and Biotechnology
|June 10, 2025
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Summary

This study engineered Aspergillus oryzae for enhanced ergothioneine (EGT) production. Supplementing glucose and methionine significantly boosted EGT yields, offering a novel strategy for efficient synthesis of this antioxidant amino acid.

Keywords:
Agrobacterium transformationAspergillus oryzaeNeurospora crassaErgothioneineFilamentous fungus

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Area of Science:

  • Biotechnology
  • Mycology
  • Biochemistry

Background:

  • Ergothioneine (EGT) is a rare amino acid with significant antioxidant and anti-inflammatory properties.
  • EGT has diverse applications in food, cosmetics, and medicine.
  • Developing efficient production methods for EGT is crucial.

Purpose of the Study:

  • To engineer Aspergillus oryzae as a high-yield host for ergothioneine (EGT) biosynthesis.
  • To characterize endogenous genes involved in EGT production in A. oryzae.
  • To optimize EGT production through genetic engineering and media supplementation.

Main Methods:

  • Engineering Aspergillus oryzae for enhanced ergothioneine (EGT) production.
  • Characterization of two endogenous homologs, AoEgt1 (vacuolar) and AoEgt2 (peroxisomal), involved in EGT biosynthesis.
  • Overexpression of EGT biosynthetic genes and optimization of culture conditions using glucose and methionine.

Main Results:

  • Identification and characterization of AoEgt1 and AoEgt2, contributing to EGT biosynthesis.
  • Overexpression of EGT genes resulted in EGT yields of 15.17 mg/g dry weight.
  • Supplementation with glucose and methionine increased EGT production to 20.03 mg/g dry weight, an eight-fold increase over the wild-type.

Conclusions:

  • Successful construction of a high-yielding A. oryzae strain for ergothioneine (EGT) biosynthesis.
  • Demonstrated the contribution of AoEgt1 and AoEgt2 to EGT production.
  • Established a novel and efficient strategy for ergothioneine synthesis.