Related Experiment Video
Updated: Jun 12, 2025
A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
Published on: June 5, 2019
A study of soluble CD30 in patients with primary immune thrombocytopenia
Amira M N Abdelrahman1, Yasser Mahmoud Ismail1, Aliaa Mohammed Diab2
1Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt.
Introduction:
The primary cause of immune thrombocytopenic purpura (ITP), an acquired type of thrombocytopenia, is the destruction of platelets by autoantibodies. Nowadays, there is a lot of interest in using noninvasive biomarkers to diagnose and detect the severity of many disorders. This study aimed to evaluate the association of one such biomarker, CD30, with primary immune thrombocytopenia.
Methods:
The study included 50 patients with ITP and 30 healthy individuals with matched age and sex used as a control group. For all participants, the messenger RNA (mRNA) expression of CD30 was measured by real-time polymerase chain reaction, and the concentration of plasma soluble CD30 (sCD30) was estimated using enzyme-linked immunosorbent assay.
Results:
The plasma sCD30 level was substantially higher in patients with ITP than in control individuals (P = .043), and the expression level of CD30 mRNA was substantially higher in patients with ITP than in control individuals (P < .001). In addition, the plasma sCD30 level was higher in active ITP cases than in remission ITP cases (P < .001), and the expression of CD30 mRNA was substantially higher in active ITP cases than in remission ITP cases (P < .001).
Discussion:
Estimation of plasma sCD30 and CD30 mRNA levels has good utility for assessing the disease activity in patients with ITP.
More Related Videos
12:36Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
07:39Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Published on: January 7, 2019