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FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 12, 2025

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Visualizing chromatin communication in living cells through Oligo-LiveFISH.

Mattia Conte¹, Mario Nicodemi¹

¹Dipartimento di Fisica, Università di Napoli Federico II and INFN Napoli, Complesso Universitario di Monte Sant'Angelo, 80126 Naples, Italy.

Cell Reports Methods

|June 10, 2025

Summary

Researchers developed Oligo-LiveFISH, a novel live-cell imaging technique. This method tracks genome organization dynamics with high resolution without requiring genetic modification.

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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

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Last Updated: Jun 12, 2025

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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Understanding genome regulation necessitates real-time observation of chromatin dynamics.
Current methods for tracking genomic loci in live cells often require genome engineering, limiting their application.

Purpose of the Study:

To introduce Oligo-LiveFISH, a new platform for high-resolution live-cell imaging of chromatin dynamics.
To enable tracking of non-repetitive genomic loci without the need for genome engineering.

Main Methods:

Assembling fluorescent guide RNA pools with catalytically deactivated Cas9 (dCas9).
Utilizing the assembled complexes for high-resolution imaging of specific genomic loci in live cells.
Achieving 20-nm spatial and 50-ms temporal resolution.

Main Results:

Demonstrated the capability of Oligo-LiveFISH to track non-repetitive genomic loci in real time.
Successfully applied the platform across various cell lines and primary cells.
Provided insights into the dynamic organization of the genome.

Conclusions:

Oligo-LiveFISH offers a powerful, non-invasive tool for studying genome regulation and dynamics.
The platform's high resolution and versatility advance the field of live-cell imaging for genomic studies.
This method facilitates a deeper understanding of dynamic genome organization in different cellular contexts.