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Simultaneous Detection of MAO-B and Soluble Aβ Oligomers Based on a Bimetallic Loaded ZIF-8 Core-Shell Probe
Yunfei Tang1, Mengmeng Xiao1, Tongtong Duo1
1Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
Abstract:
The abnormal expression of monoamine oxidase B (MAO-B) and the soluble oligomer (Aβo) formed by β-amyloid deposition play key roles in the pathogenesis of Alzheimer's disease. However, the simultaneous detection of MAO-B and Aβo remains a huge challenge. Herein, a bimetallic core-shell probe ZIF-8 embedding rhodamine 6G (Au/Rh6G/Pd@ZIF-8) with peroxidase-like activity is designed to detect MAO-B and Aβo simultaneously. MAO-B catalyzes the substrate phenethylamine (PEA) to produce H2O2, which oxidizes thiamine with the catalysis of Au/Rh6G/Pd@ZIF-8 to produce a fluorescent product that emits a strong fluorescence at 440 nm. When Aβo is present in the system, due to the specific coordination between Aβo and Zn2+, the intact ZIF-8 structure collapses, leading to Rh6G release and fluorescence recovery. The simultaneous determination of MAO-B and Aβo by Au/Rh6G/Pd@ZIF-8 is realized. The results showed that the designed biosensor had high specificity and sensitivity, a wide detection range, and a low detection limit for MAO-B and Aβo simultaneous detection. Fluorescence signals for MAO-B and Aβo detection did not interfere with each other because they are excited separately, which provides more valuable data for the diagnosis of Alzheimer's disease.
Insights
A novel biosensor simultaneously detects monoamine oxidase B (MAO-B) and amyloid-beta oligomers (Aβo), key in Alzheimer's disease. This breakthrough offers improved diagnostic data by overcoming previous detection challenges.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Neuroscience
Background:
- Abnormal monoamine oxidase B (MAO-B) and amyloid-beta oligomers (Aβo) are crucial in Alzheimer's disease pathogenesis.
- Simultaneous detection of MAO-B and Aβo presents a significant analytical challenge.
Purpose of the Study:
- To develop a novel bimetallic core-shell probe for the simultaneous detection of MAO-B and Aβo.
- To establish a sensitive and specific method for Alzheimer's disease biomarker quantification.
Main Methods:
- Design of a Au/Rh6G/Pd@ZIF-8 core-shell probe with peroxidase-like activity.
- Utilizing MAO-B's catalytic production of H2O2 to generate fluorescence via thiamine oxidation.
- Leveraging Aβo-induced ZIF-8 structural collapse for fluorescence recovery detection.
Main Results:
- The Au/Rh6G/Pd@ZIF-8 probe achieved simultaneous detection of MAO-B and Aβo.
- The biosensor demonstrated high specificity, sensitivity, a wide detection range, and a low detection limit.
- Independent fluorescence signals for MAO-B and Aβo ensured no cross-interference.
Conclusions:
- The developed bimetallic probe offers a promising tool for the simultaneous quantification of MAO-B and Aβo.
- This method provides valuable data for the diagnosis and monitoring of Alzheimer's disease.
- The probe's design overcomes previous limitations in simultaneous biomarker detection.
