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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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  9. Predictive And Prognostic Markers
  11. Single‑cell Transcriptomic Analysis Revealed The Tumor‑associated Microenvironment Of Papillary Thyroid Carcinoma With Metastasis

Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis

Ni Zhang1, Qingbin Liu2, Qian Wang3

  • 1Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250000, P.R. China.

Oncology Letters
|June 11, 2025

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Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

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View abstract on PubMed

Summary
This summary is machine-generated.

Single-cell RNA sequencing reveals immune cell differences in metastatic papillary thyroid cancer (PTC). Immunosuppressive cells like M2 macrophages, DC2s, and Tregs correlate with metastasis, suggesting new therapeutic targets.

Area of Science:

  • Immunology
  • Oncology
  • Genomics

Background:

  • Papillary thyroid cancer (PTC) often involves inflammation and lymph node metastasis.
  • Single-cell RNA sequencing (scRNA-seq) offers high-resolution analysis of cellular heterogeneity within tumor microenvironments.

Purpose of the Study:

  • To investigate immune cell population differences in metastatic PTC (PTC-M) compared to normal tissues and non-metastatic PTC using scRNA-seq.
  • To identify specific immune cell subsets associated with PTC metastasis and tumor progression.

Main Methods:

  • Utilized scRNA-seq to analyze immune cells (macrophages, dendritic cells, T cells) in metastatic PTC, non-metastatic PTC, and adjacent normal tissues.
  • Compared immune cell composition between different tissue types to identify significant variations.
Keywords:
dendritic cellmicroenvironmentpapillary thyroid cancersingle-cell RNA sequencing

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Main Results:

  • Identified significant immune cell heterogeneity in PTC-M, indicating an immunosuppressive tumor microenvironment.
  • Found that alternatively activated M2 macrophages, conventional type 2 dendritic cells (DC2s), and regulatory T cells (Tregs) are associated with increased lymph node metastasis and advanced cancer stage.
  • Observed that monocytes and B cells may play a protective role against tumors.
  • Characterized a subset of tumor-associated DC2s expressing LAMP3 and CCL22, capable of attracting CD4+ T cells.

Conclusions:

  • The study supports the hypothesis that myeloid cells and Tregs significantly contribute to PTC progression and metastasis by modulating the tumor microenvironment.
  • The findings highlight potential therapeutic strategies targeting these immune cells for PTC treatment.