Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments.

Molecular medicine reports·2017

Same author

A PDGFB mutation causes paroxysmal nonkinesigenic dyskinesia with brain calcification.

Movement disorders : official journal of the Movement Disorder Society·2017

Same author

A Molecular Switch Regulating Cell Fate Choice between Muscle Progenitor Cells and Brown Adipocytes.

Developmental cell·2017

Same author

Microarray analysis of differentially expressed genes and their functions in omental visceral adipose tissues of pregnant women with vs. without gestational diabetes mellitus.

Biomedical reports·2017

Same author

Development and validation of a simplified titration method for monitoring volatile fatty acids in anaerobic digestion.

Waste management (New York, N.Y.)·2017

Same author

Association Analysis of Nonsyndromic Congenital Heart Disease and Tag Single Nucleotide Polymorphisms of TBX20 and Genes in the Ras-MAPK Pathway.

Genetic testing and molecular biomarkers·2017

Search research articles

Related Experiment Video

Updated: Jun 13, 2025

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

Deciphering mitochondrial dysfunction in keratoconus: Insights into ACSL4 from machine learning-based bulk and

Yuchen Cai^1,2, Tianyi Zhou^1,2, Xueyao Cai³

¹Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Computational and Structural Biotechnology Journal

|June 11, 2025

Summary

Mitochondrial dysfunction plays a key role in keratoconus (KC), a leading cause of corneal transplants. The study identifies ACSL4 as a crucial biomarker linked to KC development and potential therapeutic strategies.

Keywords:

ACSL4 Corneal stromal cells Keratoconus Mitochondrial dysfunction Single cell transcriptome

More Related Videos

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Published on: November 12, 2015

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Published on: January 22, 2017

Related Experiment Videos

Last Updated: Jun 13, 2025

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Published on: November 12, 2015

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Published on: January 22, 2017

Area of Science:

Ophthalmology
Molecular Biology
Genetics

Background:

Keratoconus (KC) is a major cause of corneal transplantation worldwide.
Mitochondrial abnormalities are observed in KC, but mechanisms are poorly understood.

Purpose of the Study:

To investigate the role of mitochondrial dysfunction in keratoconus pathogenesis.
To identify key molecular players and potential biomarkers for KC.

Main Methods:

Transcriptomics analysis of KC datasets to identify mitochondria-related differentially expressed genes (mDEGs).
Functional pathway analysis, protein-protein interaction (PPI) network construction, and machine learning (ML) for hub gene screening.
Validation using quantitative PCR, Western blot, and an in vitro KC model.

Main Results:

104 mDEGs were identified, enriched in pathways of oxidative stress, apoptosis, ferroptosis, and inflammation.
Nine characteristic genes were validated, with ACSL4 identified as a promising biomarker, particularly in corneal stromal cells (CSCs).
ACSL4 expression increased with decreased substrate stiffness in vitro and correlated with immune cell infiltration in KC.

Conclusions:

Mitochondrial dysfunction, particularly involving ACSL4, is implicated in keratoconus pathogenesis.
ACSL4 serves as a potential novel biomarker for understanding KC mechanisms and developing future therapies.