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Updated: Jun 12, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

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Aptasensing Technology and Its Potential Applications: Where Do We Stand?

Jhilik Roy^1,2, Neelanjana Bag¹, Shubham Roy³

¹Department of Physics, Jadavpur University, Kolkata 700032, India.

Molecular Pharmaceutics

|June 11, 2025

Summary

This summary is machine-generated.

Aptasensing technologies offer versatile applications in diverse fields, from clinical diagnostics to environmental monitoring. This review highlights their potential, discusses mechanisms, and proposes solutions to overcome challenges for broader adoption.

Keywords:

SELEX aptamer aptasensors artificial intelligence diagnosis nanoparticles

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Area of Science:

Biomedical Engineering
Materials Science
Clinical Microbiology
Food Science

Background:

Aptamers offer stability, target affinity, and modifiability, advancing biosensing.
Key challenges currently limit the practical translation of aptasensing technologies into widespread platforms.

Purpose of the Study:

To explore the future potential of aptasensing technologies across diverse fields.
To discuss underlying molecular mechanisms, applications, and effectiveness of aptasensors.
To propose solutions for existing challenges and diversify aptasensor applications.

Main Methods:

Comprehensive literature review of aptasensing technologies.
Analysis of aptamer mechanisms, applications, and effectiveness.
Identification and discussion of challenges and potential solutions in aptasensing.

Main Results:

Aptasensing holds significant future potential in clinical chemistry, quality control, protein analysis, wastewater treatment, nanomaterial characterization, forensic analysis, animal health, heavy metal detection, and security.
Machine learning and AI are emerging as key tools in aptamer design.
Numerous challenges were identified, with proposed solutions to facilitate future aptasensing adoption.

Conclusions:

Aptasensing technologies are poised for advancement toward clinical applications.
Addressing current challenges will expedite the selection and diversification of aptasensing paradigms.
This review provides critical updates for researchers in relevant scientific disciplines.