Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

297
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
297

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic silencing of miR-141 via core promoter methylation is associated with short-term bladder cancer progression.

The Journal of biological chemistry·2026
Same author

Epirus-yellow (EY): A dual naked-eye colorimetric and NIR fluorimetric chemosensor for the rapid, sensitive, and selective detection of Sn<sup>2</sup>.

Food chemistry·2026
Same author

The IKKα-regulated microRNA miR-9-5p mediates lung cancer growth and invasiveness via CDH1/Wnt/β-catenin signalling.

Cell death discovery·2026
Same author

The Protein Phosphatase Inhibitor LB100 Targets the Mesenchymal Lineage of Pancreatic Ductal Adenocarcinoma.

MedComm·2026
Same author

Real-time fidelity assessment of fluorescence molecular imaging without reference images.

Journal of biomedical optics·2026
Same author

Workshop on Noninvasive Glucose Monitoring 2025.

Journal of diabetes science and technology·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2025

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.1K

Probing Field Cancerization in the Gastrointestinal Tract Using a Hybrid Raman and Partial Wave Spectroscopy

Elena Kriukova1,2, Mikhail Mazurenka1,2, Sabrina Marcazzan1,2

  • 1Chair of Biological Imaging, Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine and Health & School of Computation, Information and Technology, Technical University of Munich, Munich 81675, Germany.

Analytical Chemistry
|June 11, 2025
PubMed
Summary
This summary is machine-generated.

Field cancerization (FC) detection is improved using combined Raman and partial wave spectroscopy (RS-PWS). This hybrid approach identifies molecular and morphological changes in precancerous tissue, enhancing early cancer screening capabilities.

More Related Videos

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.1K
Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

7.8K

Related Experiment Videos

Last Updated: Jun 12, 2025

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.1K
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.1K
Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

7.8K

Area of Science:

  • Biophotonics and Spectroscopy
  • Cancer Research
  • Molecular Pathology

Background:

  • Field cancerization (FC) describes premalignant tissue changes preceding malignancy, crucial for early cancer detection.
  • Existing screening methods may not fully capture subtle, spatially distributed precancerous alterations.
  • Novel optical techniques are needed to detect molecular and morphological changes in macroscopically normal tissues adjacent to tumors.

Purpose of the Study:

  • To evaluate the efficacy of combined Raman spectroscopy (RS) and partial wave spectroscopy (PWS) in detecting field cancerization.
  • To investigate the individual and combined performance of RS and PWS in identifying precancerous changes in gastroesophageal and intestinal tumor models.
  • To correlate optical findings with gene expression patterns in tumor-adjacent tissues.

Main Methods:

  • Utilized a hybrid RS-PWS microscope to acquire molecular and morphological data from macroscopically normal tumor-adjacent tissues in L2-IL1B and Villin-Cre, Apcfl/wt mouse models.
  • Employed partial least-squares discriminant analysis (PLS-DA) for data analysis.
  • Performed RNA-sequencing for transcriptomic profiling of the analyzed tissues.

Main Results:

  • RS detected significant increases in free amino acid bands and decreases in lipid and carotenoid bands in normal tissue of tumor-bearing mice compared to controls.
  • PWS data improved the classification accuracy of RS results by approximately 9% in L2-IL1B mice and 5% in Villin-Cre, Apcfl/wt mice.
  • Transcriptomic analysis revealed significant correlations between observed optical changes and gene expression profiles.

Conclusions:

  • Combined RS-PWS effectively detects molecular and morphological alterations indicative of field cancerization in precancerous tissues.
  • The synergistic use of molecular (RS) and structural (PWS) information enhances the sensitivity for identifying early neoplastic changes.
  • This integrated spectroscopic approach offers valuable insights into tissue alterations during cancer development and holds promise for improved cancer screening.