Neomycin loaded by tetrahedral framework nucleic acids enhances antimicrobial sensitivity against bacteria

Area of Science:

• Biomaterials Science
• Nanotechnology
• Infectious Disease Research

Background:

• Burn wound infections present significant clinical challenges.
• Neomycin sulfate (NeoS) is widely used but has limitations like low bioavailability and side effects.
• Improved therapeutic strategies for NeoS are urgently needed.

Purpose of the Study:

• To develop a novel drug delivery system for neomycin sulfate (NeoS) using tetrahedral framework nucleic acids (tFNAs).
• To evaluate the efficacy of tFNAs-loaded NeoS (tFNA-NeoS) in enhancing antibiotic sensitivity and promoting wound healing.

Main Methods:

• Fabrication of tFNAs-loaded NeoS (tFNA-NeoS) nanoparticles.
• In vitro assessment of antibiotic sensitivity against *Escherichia coli* and *Staphylococcus aureus*.
• In vivo studies in a rat burn wound infection model.

Main Results:

• tFNA-NeoS demonstrated enhanced sensitivity to target bacteria, allowing for reduced antibiotic dosage.
• tFNA-NeoS exhibited improved stability, biocompatibility, and tissue utilization compared to free NeoS.
• In vivo results showed tFNA-NeoS possesses anti-inflammatory properties, activates angiogenesis, and accelerates wound healing.

Conclusions:

• The tFNAs-based delivery system effectively enhances NeoS efficacy and reduces side effects.
• This strategy holds significant promise for improving burn wound management and broader antibacterial applications.