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In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
Expression of the Transcription Factor FOXP3 in Human Peripheral Blood B-Cell Subtypes (CD19+CD39+ and CD19+CD39-)
A Cardenas-Juarez1, E E Uresti-Rivera2,3, F Ochoa-González4
1Unidad de Investigación Biomédica, Delegación Zacatecas, Instituto Mexicano del Seguro Social, IMSS.
Introduction:
The aim of this study was to evaluate FOXP3 expression in CD19+CD39+ and CD19+CD39- B cells, and to investigate its potential regulatory role.
Methods:
Peripheral B cells were obtained from 25 volunteers. FOXP3 expression at the mRNA and protein levels was analyzed in CD19+CD39+ and CD19+CD39- B cells by FACS and RT-qPCR. Suppressive activity was assessed through co-cultures of PBMC with CD19+CD39+ and CD19+CD39- B cells stimulated with anti-CD3/CD28, evaluating T cell proliferation and the percentage of Th1 cells.
Results:
The percentage of CD19+CD39+ FOXP3+ B cells was higher compared to other phenotypes. There was a positive correlation between FOXP3 and CD39 in CD19+ B cells. FOXP3 mRNA was increased in CD19+CD39+ B cells compared to CD19+CD39- B cells. CD19+CD39- B cells reduced the proliferation, the percentage of Th1 cells, and expressed higher IL-10 mRNA compared to CD19+CD39+ B cells. B cell phenotypes were inversely associated with Th1 cells and CRP. CD19+CD39- was associated with HOMA-β. CD19+CD39+ was inversely associated with HbA1c.
Discussion:
FOXP3 is expressed on both CD19+CD39- and CD19+CD39+ B lymphocytes. CD19+CD39- cells showed high levels of IL-10 and low levels of FOXP3 mRNA. CD19+CD39- B cells decreased the Th1 cells and were associated with β-cell function.
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